|
KMID : 0366119740020010037
|
|
Korean Journal of Applied Microbiology & Bioengineering
1974 Volume.2 No. 1 p.37 ~ p.43
|
|
|
Studies on Cysteinedesulfhydrase produced by Bacteria
|
|
|
|
|
Abstract
|
|
|
1. In the course of investigation on the metabolism of cysteine by microorganisms, the authors have found that among 70 strains tested Cysteinedesulfhydrase occured most remarkably in the cells of the strain I-3-2 isolated from the soil when it was grown on a medium containing L-Cysteine.
The morphological, cultural and physiological properties of this strain were investigated. From the results, the bacterium was identified as a variety of Aerobactor aerogenes.
2. The cultural conditions for the formation of Cysteinedesulfhydrase by the strain I-3-2 were also investigated and the results were as follows:
1). The optium pH of the culture medium was 7.0―7.5. 2). As a carbon source, glycerol was most effective when it was added to the basal medium at 0.1 % concentration.
3). By addition of calcium chloride at 0.2% concentration, the formation of the enzyme remarkably increased. 4). Maximal formation of the enzyme was observed at the end of
logarithmic phase of cell growth, thereafter the enzyme activity was diminished rapidly.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|